Optical / X-ray Imaging

Animal and Probe Preparation for Imaging

Optical imaging using fluorescent agents requires proper preparation of both the animal and the fluorescent probe to achieve accurate and high-quality images. For animal preparation, this may include proper positioning, immobilization, and grooming to reduce movement during the scan. The preparation of the fluorescent probe may include proper dilution, administration, and time to allow for optimal accumulation in the target tissue.

Fluorescent Agent

The selection of an appropriate fluorescent agent is crucial in optical imaging. The choice of fluorescent agent can affect the quality of the images, including brightness, specificity, and stability. Factors such as the type of target molecule, imaging environment, and the intended use of the images must be taken into consideration when selecting a fluorescent agent.

Scan Parameters Selection

The selection of appropriate scan parameters is a crucial part of optical imaging using fluorescent agents. The excitation and emission wavelength, as well as the filters used, play a significant role in determining the quality and specificity of the images. The distance between the animal and the camera, the animal’s position, and the scan duration are also important factors to consider in order to achieve the desired imaging goals. Careful consideration of these parameters, as well as a thorough understanding of the sample or animal being imaged, is essential for obtaining high-quality images with optimal resolution.

Scan and Image Analysis

The final step in the optical imaging procedure is capturing the images and analyzing them. This process involves illuminating the sample with specific wavelengths of light, and then capturing the emitted fluorescence with a specialized camera. Image analysis is then performed to extract meaningful information about the sample, such as the distribution and localization of the fluorescent agents, and changes in fluorescence intensity over time. Our team of experts use advanced software and techniques to carefully analyze the images and provide valuable insights into the biological processes being studied. This information can be used to support research and development, quality control, and clinical decision-making.